Part:BBa_K3635013:Design
T7 promoter+RBS+lac operator+Tyrosinase+7xhis-tag+pBluescript II SK+ f1 ori+AmpR
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 564
Design Notes
The T7 promoter has been used for the timely induction of both expression vectors to ensure in vivo polymerisation occurs parallel to protein production. It is essential that both plasmids in the co-expression system are working under the same promoter as if Pvfp-5 Beta is expressed into the cytoplasm with no tyrosinase present, tyrosine residues will be converted to dopamine. These dopamine molecules will then undergo irreversible spontaneous oxidation which will severely limit adhesion. This tyrosinase enzyme must be expressed in ORF438 to ensure that copper atoms can incorporate at the active site, This will give optimum enzyme activity.
Source
This part uses BBa_K3635008 and BBa_K3033013